One-Step RT-PCR Master Mix

Attributes

  • product-brand: biotechrabbit Germany

One-Step RT-PCR Kit from Biotech Rabbit Germany | Ideal for Viral Detection and Complex Templates

The One-Step RT-PCR Kit from Biotech Rabbit is a high-quality product from the well-known German brand Biotech Rabbit, designed for simultaneous reverse transcription and PCR in a single tube – ideal for rapid and reliable RNA analysis.

Key Features One-Step RT-PCR Master Mix:

  • Contains heat-stable reverse transcriptase and RNase inhibitor for efficient and consistent high-yield cDNA synthesis

  • Includes Hot-start Taq DNA Polymerase combined with high-purity dNTPs to ensure high fidelity and reproducibility

  • Enhanced with special PCR additives for ultra-sensitive amplification with minimal background noise, even for GC-rich or complex genetic templates

Applications One-Step RT-PCR Master Mix:

  • One-step RT-PCR reactions

  • Rapid and accurate detection of RNA viruses in clinical samples

  • Robust amplification of GC-rich and complex RNA templates

🧪 This kit is certified with international quality standards and is widely used in advanced molecular diagnostics laboratories.

📌 Fardad Azma Rad Co. is the exclusive distributor of Biotech Rabbit in Iran, providing official access to this kit and other molecular diagnostic products in the region.

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Overview:

Biotechrabbit™ 1-Step RT-PCR Mix offers an easy and efficient solution for simultaneous reverse transcription (RT) of RNA and PCR amplification of cDNA in a single step. Simply add RNA samples, primers, and PCR water to proceed.

Key Components:

  • RT-RI 20× Mix: Contains stable reverse transcriptase able to operate at different temperatures, along with a specific RNase inhibitor, ensuring high-performance cDNA synthesis.
  • 2X 1-Step Master Mix: Combines hot-start Taq DNA Polymerase, high-quality dNTPs, MgCl₂, and stabilizing agents in an optimized buffer. It produces high yields of PCR products with minimal background, even from low-abundance or challenging templates.

Benefits:

  • Sensitive detection of difficult or GC-rich targets
  • Minimal background noise for high-specificity results
  • Rapid, one-tube workflow suitable for diagnostic labs, research, and pathogen detection

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