Main features Detection of the main mutations of exon 2 (codons 12,13), of exon 3 (codons 61) of the genes KRAS, NRAS, HRAS and of the codons 600 and 601 of the gene BRAF using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene.
Controls Controls Positive control DNA containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
Starting material The kit allows the analysis of genomic DNA extracted from fresh, frozen or formalin fixed paran-embedded (FFPE) tumor tissue. The kit allows the analysis of genomic DNA extracted from cytological samples.
Execution time 2 hours.
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