Main features Detection of the main mutations of codon 600 of the gene BRAF using 5 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample. Control DNA positive for all the mutations detected by the kit. Controls Reference standard DNA Horizon BRAF V600E 1% to monitor the analytical process and the performances of the system. The standard is characterized by a well defined allelic ratio wild-type/mutant. Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAM and HEX (LOD down to 0.5%). Starting material The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded tissue. Execution time 2 hours.
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