کیت Vectors and Linear Template Kits

RTS Linear Template Kit Plus
Features
.Efficient in vitro expression (E. coli and insect kits)
.Primers for affinity tags (His6-tag /Strep-tag II)
.Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Applications
.Fast generation of multiple template constructs
.Accelerated screening of protein expression templates and conditions

RTS Linear Template Fab Kit
Features
.Introduction of an affinity tag for protein purification
.Signal peptide sequence for translocation into microsomes
.Introduction of a signal peptide sequence for translocation into microsomes
.Compatibility with E. coli HY, Fab and Insect kits
Applications
.PCR-based inear templates for protein expression in E. coli and insect disulfide kits
.Fast template generation for expression of Fab and scFv antibody fragments

RTS 100 E. coli LinTempGen Set, His-tag
Features
.Contains all regulatory elements in optimal spacing
.Option for templates with N- or C-terminal His6-tag
.Primary PCR product can be cloned into pIVEX vectors
.For use with RTS 100 E. coli HY and insect kits
Applications
.Fast gene template generation without cloning
.Generate linear templates for rapid screening and optimization of His6-tagged protein expression

RTS Wheat Germ LinTempGen Set, His6-tag
Features
.Optimized spacing of eukaryotic regulatory elements
.Time saving, no laborious cloning
.Flexible positioning of His6-tag at the C- or N-terminus
.Primary PCR product can be cloned into pIVEX vectors
Applications
.Rapid generation of linear expression constructs for screening applications
.Linear template generation for optimized His6-tagged protein expression

RTS pIX3.0 Vector
Features
.Multiple cloning site compatible with linear templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
.High copy origin of replication
.Cell-free protein synthesis scale-up to after expression screening using linear templates
.Free of any potentially interfering regulatory elements ensuring efficient transcription and translation
.Compatible with RTS E. coli and insect kits
Applications
.Cloning of RTS Linear Templates for expression in RTS E. coli and insect kits

RTS pIX4.0 Insect Vector
Features
.Regulatory elements for efficient transcription and translation
.Multiple cloning site and translational stop codons in all reading frames
.Optimized untranslated regions stabilizing and protecting RNA from degradation by exonucleases
.RNA of defined length via optional plasmid linearization
Applications
.Cloning genes for efficient synthesis of proteins using the RTS 100 Insect kits

RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set
Features
.Stable circular templates to ensure high yields in preparative-scale expressions
.For construction of C- and N-terminal His6-tagged proteins
.Optimal spacing of all regulatory elements
.For use with RTS E. coli and Insect kits
Applications
.Preparative-scale expression of His6-tagged proteins

RTS pIVEX Wheat Germ His6-tag Vector Set
Features
.Stable circular templates ensure high yields in preparative-scale expression reactions
.For construction of C- and N-terminal His6-tag proteins
Applications
.Expression vector for preparative-scale expression in eukaryotic wheat germ lysates