Reverse Transcription & RT-PCR
RevertUP™ II Reverse Transcriptase, 200 U/µl
Features
.Improved performance for synthesis of long cDNAs (≥ ۱۹ kb)
.Excellent stability at high temperatures up to 60°C
.High sensitivity for cDNA synthesis from few copies of template
Applications
.First-strand cDNA synthesis
.Generation of labeled cDNA
.RNA analysis by primer extension
.cDNA library construction
.RT-PCR
| Purchase RevertUP™ II Reverse Transcriptase, 200 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400501 | ۱۰۰۰۰ U | 50 rxn | ۵۰ µl RevertUP II Reverse Transcriptase ۱ ml 5× RevertUP Buffer |
| BR0400502 | ۵۰۰۰۰ U | 250 rxn | ۲ × ۱۲۵ µl RevertUP II Reverse Transcriptase ۲ × ۱ ml 5× RevertUP Buffer |
MMuLV Reverse Transcriptase, 200 U/µl
Features
.Pure reverse transcriptase for cDNA synthesis
.High yields of first-strand cDNA
.High value for a fair price
Applications
.First-strand cDNA synthesis
.Generation of labeled cDNA
.RNA analysis by primer extension
| Purchase MMuLV Reverse Transcriptase, 200 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400201 | ۱۰۰۰۰ U | 50 rxn | ۵۰ µl MMuLV Reverse Transcriptase ۱۰۰ µl 10× MMuLV RT Buffer |
| BR0400202 | ۵۰۰۰۰ U | 250 rxn | ۵ × ۵۰ µl MMuLV Reverse Transcriptase ۵ × ۱۰۰ µl 10× MMuLV RT Buffer |
One Step RT PCR Kit
Features
.Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
.Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
.PCR enhancers allowing sensitive low background amplification
Applications
.One-step RT-PCR
.Virus detection
.Amplification of GC-rich and complex templates
| Purchase One Step RT PCR Kit | ||
| Catalog # | Size | Package Content |
| BR0400101 | ۵۰ rxn of 50 µl | ۱٫۲۵ ml One Step Mix ۱۲۵ µl RT-RI Blend |
| BR0400102 | ۱۰۰ rxn of 50 µl | ۲ × ۱٫۲۵ ml One Step Mix ۲ × ۱۲۵ µl RT-RI Blend |
| BR0400103 | ۵۰۰ rxn of 50 µl | ۱۰ × ۱٫۲۵ ml One Step Mix ۱۰ × ۱۲۵ µl RT-RI Blend |
cDNA Synthesis Kit
Features
.Highly efficient synthesis of long cDNAs (≥ ۱۹ kb)
.Excellent yields at temperatures up to 55°C
.High sensitivity reverse transcription from low abundance template
.Superior performance in demanding applications, including templates with a high degree of secondary structure
Applications
.First-strand cDNA synthesis for RT-PCR and qPCR
.Gene expression profiling
.RNA labeling and primer extension
.cDNA library construction
| Purchase cDNA Synthesis Kit | ||
| Catalog # | Size | Package Content |
| BR0400401 | ۱۰ rxn of 20 µl | ۱۰ µl Reverse Transcriptase ۱۰۰ µl 5× cDNA Synthesis Buffer ۲۰ µl dNTP Mix, 5 µl RNase Inhibitor ۱۰ µl Hexamer and 5 µl Oligo (dT) Primer ۱٫۵ ml PCR Grade Water |
| BR0400403 | ۱۲۵ rxn of 20 µl | ۱۲۵ µl Reverse Transcriptase ۱ ml 5× cDNA Synthesis Buffer ۲۵۰ µl dNTP Mix, 62.5 µl RNase Inhibitor ۱۲۵ µl Hexamer and 62.5 µl Oligo (dT) Primer |
RNase Inhibitor, 40 U/µl
Features
.Exceptionally pure proprietary Ribonuclease Inhibitor for demanding RNA applications
.Active under variety of reaction conditions used for work with RNA
.Prevention of RNA from degradation by a wide range of RNases
Applications
.In vitro transcription/translation
.cDNA synthesis
.RNA purification and storage
| Purchase RNase Inhibitor, 40 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400901 | ۲۵۰۰ U | 125 rxn | ۶۲٫۵ µl RNase Inhibitor |
| BR0400902 | ۱۰۰۰۰ U | 500 rxn | ۴ × ۶۲٫۵ µl RNase Inhibitor |