Hot Start PCR
۱
Hot Start Taq DNA Polymerase, 5 U/µl
Features
.High PCR specificity and sensitivity
.Exceptionally pure Hot Start Taq DNA Polymerase for sensitive PCR applications and high yields
.Antibody-based Hot Start for fast polymerase activation
Applications
.Hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase Hot Start Taq DNA Polymerase, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0200101 | ۱۰۰ U | ۲۰ µl Hot Start Taq DNA Polymerase ۱٫۵ ml 10× Reaction Buffer ۱٫۵ ml 50 mM MgCl2 ۱٫۵ ml 5× PCR Enhancer |
| BR0200102 | ۵۰۰ U | ۱۰۰ µl Hot Start Taq DNA Polymerase ۲ × ۱٫۵ ml 10× Reaction Buffer ۱٫۵ ml 50 mM MgCl2 ۱٫۵ ml 5× PCR Enhancer |
| BR0200103 | ۲۵۰۰ U | ۵۰۰ µl Hot Start Taq DNA Polymerase ۱۰ × ۱٫۵ ml 10× Reaction Buffer ۵ × ۱٫۵ ml 50 mM MgCl2 ۱٫۵ ml 5× PCR Enhancer |
۲
Hot Start Taq DNA Polymerase, convenient, 5 U/µl
Features
.Optimal concentration of MgCl2 included in Reaction Buffer
.High-sensitivity and robustness in a wide application range
.Exceptionally pure Hot Start Taq DNA Polymerase for both routine and demanding PCR applications
Applications
.High-specificity hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase Hot Start Taq DNA Polymerase, convenient, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0200601 | ۱۰۰ U | ۲۰ µl Hot Start Taq DNA Polymerase ۱٫۸ ml 10× Reaction Buffer with MgCl2 ۱٫۵ ml 5× PCR Enhancer |
| BR0200602 | ۵۰۰ U | ۱۰۰ µl Hot Start Taq DNA Polymerase ۲ × ۱٫۸ ml 10× Reaction Buffer with MgCl2 ۱٫۵ ml 5× PCR Enhancer |
| BR0200603 | ۲۵۰۰ U | ۵۰۰ µl Hot Start Taq DNA Polymerase ۷ × ۱٫۸ ml 10× Reaction Buffer with MgCl2 ۱٫۵ ml 5× PCR Enhancer |
۳
Hot Start Taq DNA Polymerase, lyophilized, 5 U/µl
Features
.Room-temperature stable enzymes and mixes
.Optimal concentration of MgCl2 included in Reaction Buffer
.High-sensitivity and robustness in a wide application range
.Exceptionally pure Hot Start Taq DNA Polymerase for both routine and demanding PCR applications
Applications
.Ambient shipment and room-temperature storage
.High-specificity hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase Hot Start Taq DNA Polymerase, lyophilized, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0201202 | ۵۰۰ U | Lyo Hot Start Taq DNA Polymerase ۱۰۰ µl Taq Reconstitution Buffer ۲ × ۱٫۵ ml 10× Reaction Buffer with MgCl2 |
| BR0201203 | ۲۵۰۰ U | ۵ × Lyo Hot Start Taq DNA Polymerase ۵ × ۱۰۰ µl Taq Reconstitution Buffer ۱۰ × ۱٫۵ ml 10× Reaction Buffer with MgCl2 |
۴
Hot Start PCR Master Mix, 2×
Features
.Highest PCR sensitivity without prolonged reactivation
.Optimized PCR Master Mix for minimal hands-on and fast setup
.Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Applications
.High-specificity and high-throughput hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.TA cloning
| Purchase Hot Start PCR Master Mix, 2× | ||
| Catalog # | Size | Package Content |
| BR0200201 | ۱۰۰ rxn of 50 µl | ۲ × ۱٫۲۵ ml Hot Start PCR Master Mix ۱٫۵ ml 5× PCR Enhancer |
| BR0200202 | ۵۰۰ rxn of 50 µl | ۱۰ × ۱٫۲۵ ml Hot Start PCR Master Mix ۱٫۵ ml 5× PCR Enhancer |
| BR0200204 | ۲۰۰۰ rxn of 50 µl | ۴۰ × ۱٫۲۵ ml Hot Start PCR Master Mix ۵ × ۱٫۵ ml 5× PCR Enhancer |
۵
Hot Start PCR Master Mix, lyophilized, 2×
Features
.Room-temperature stable enzymes and mixes
.Exceptionally pure Taq DNA Polymerase
.Hot-start for high PCR specificity and sensitivity
.Optimized Master Mix for fast setup
Applications
.Ambient shipment and room-temperature storage
.Routine and demanding PCR applications
.PCR amplification up to 5 kb
.TA cloning
| Purchase Hot Start PCR Master Mix, lyophilized, 2× | ||
| Catalog # | Size | Package Content |
| BR0201101 | ۵۰ rxn of 50 µl | Lyo Hot Start PCR Master Mix ۱٫۲۵ ml PCR Reconstitution Buffer |
| BR0201102 | ۲۵۰ rxn of 50 µl | ۵ × Lyo Hot Start PCR Master Mix ۵ × ۱٫۲۵ ml PCR Reconstitution Buffer |
۶
UPta™ Taq DNA Polymerase, 5 U/µl
Features
.Aptamer-based hot-start functionality
.No activation steps needed
.High PCR specificity and sensitivity
.Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
Applications
.Fast PCR reactions
.Hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase UPta™ Taq DNA Polymerase, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0200901 | ۱۰۰ U | ۲۰ µl UPta Taq DNA Polymerase ۱٫۵ ml 10× UPta Taq Reaction Buffer ۱٫۵ ml 50 mM MgCl2 |
| BR0200902 | ۵۰۰ U | ۱۰۰ µl UPta Taq DNA Polymerase ۲ × ۱٫۵ ml 10× UPta Taq Reaction Buffer ۱٫۵ ml 50 mM MgCl2 |
| BR0200903 | ۲۵۰۰ U | ۵ × ۱۰۰ µl UPta Taq DNA Polymerase ۱۰ × ۱٫۵ ml 10× UPta Taq Reaction Buffer ۵ × ۱٫۵ ml 50 mM MgCl2 |
۷
UPta™ Taq DNA Polymerase, lyophilized, 5 U/µl
Features
.Room-temperature stable enzymes and mixes
.Aptamer-based hot-start functionality – no activation steps needed
.High PCR specificity and sensitivity
.Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
Applications
.Ambient shipment and room-temperature storage
.Fast PCR reactions
.Hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase UPta™ Taq DNA Polymerase, lyophilized, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0201402 | ۵۰۰ U | Lyo UPta Taq DNA Polymerase ۱۰۰ µl Taq Reconstitution Buffer ۲ × ۱٫۵ ml 10× Reaction Buffer with MgCl2 |
| BR0201403 | ۲۵۰۰ U | ۵ × Lyo UPta Taq DNA Polymerase ۵ × ۱۰۰ µl Taq Reconstitution Buffer ۱۰ × ۱٫۵ ml 10× Reaction Buffer with MgCl2 |
۸
UPta™ Taq PCR Master Mix, 2×
Features
.Aptamer-based hot-start functionality
.Highest PCR sensitivity without any activation step
.Optimized Master Mix including exceptionally pure UPta Taq DNA Polymerase and highest quality dNTPs
Applications
.Fast PCR reactions
.High-specificity and high-throughput hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.TA cloning
| Purchase UPta™ Taq PCR Master Mix, 2× | ||
| Catalog # | Size | Package Content |
| BR0201001 | ۱۰۰ rxn of 50 µl | ۲ × ۱٫۲۵ ml UPta Taq PCR Master Mix |
| BR0201002 | ۵۰۰ rxn of 50 µl | ۱۰ × ۱٫۲۵ ml UPta Taq PCR Master Mix |
| BR0201004 | ۲۰۰۰ rxn of 50 µl | ۴۰ × ۱٫۲۵ ml UPta Taq PCR Master Mix |
۹
UPta™ Taq PCR Master Mix, lyophilized, 2×
Features
.Room-temperature stable enzymes and mixes
.Aptamer-based hot-start functionality
.Highest PCR sensitivity without any activation step
.Optimized Master Mix including exceptionally pure UPta Taq DNA Polymerase and highest quality dNTPs
Applications
.Ambient shipment and room-temperature storage
.Fast PCR reactions
.High-specificity and high-throughput hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.TA cloning
| Purchase UPta™ Taq PCR Master Mix, lyophilized, 2× | ||
| Catalog # | Size | Package Content |
| BR0201301 | ۵۰ rxn of 50 µl | Lyo UPta Taq PCR Master Mix ۱٫۲۵ ml PCR Reconstitution Buffer |
| BR0200102 | ۲۵۰ rxn of 50 µl | ۵ × Lyo UPta Taq PCR Master Mix ۵ × ۱٫۲۵ ml PCR Reconstitution Buffer |
۱۰
Green Hot Start Taq DNA Polymerase Direct-Load, 5 U/µl
Features
.Green Reaction Buffer formulation for direct loading on the gel right after PCR
.High PCR specificity and sensitivity
.Hot Start Taq DNA Polymerase for demanding sensitive PCR applications and high yields
Applications
.High-sensitivity PCR and immediate gel analysis
.High-specificity hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.RT-PCR and TA cloning
| Purchase Green Hot Start Taq DNA Polymerase Direct-Load, 5 U/µl | ||
| Catalog # | Size | Package Content |
| BR0200701 | ۱۰۰ U | ۲۰ µl Hot Start Taq DNA Polymerase ۱٫۸ ml 5× Green Direct-Load Reaction Buffer ۱٫۵ ml 50 mM MgCl2 |
| BR0200702 | ۵۰۰ U | ۱۰۰ µl Hot Start Taq DNA Polymerase ۳ × ۱٫۸ ml 5× Green Direct-Load Reaction Buffer ۱٫۵ ml 50 mM MgCl2 |
| BR0200703 | ۲۵۰۰ U | ۵۰۰ µl Hot Start Taq DNA Polymerase ۱۴ × ۱٫۸ ml 5× Green Direct-Load Reaction Buffer ۵ × ۱٫۵ ml 50 mM MgCl2 |
۱۱
Green Hot Start PCR Master Mix Direct-Load, 2×
Features
.Optimized Green Hot Start PCR Master Mix for fast setup and direct loading on the gel
.Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
.High product yields and robustness in a wide application range
Applications
.High-throughput hot-start PCR and immediate gel analysis
.High-specificity hot-start PCR up to 5 kb
.Amplification of low-copy-number targets
.TA cloning
| Purchase Green Hot Start PCR Master Mix Direct-Load, 2× | ||
| Catalog # | Size | Package Content |
| BR0200501 | ۱۰۰ rxn of 50 µl | ۲ × ۱٫۲۵ ml Green Hot Start PCR Master Mix |
| BR0200502 | ۵۰۰ rxn of 50 µl | ۱۰ × ۱٫۲۵ ml Green Hot Start PCR Master Mix |
| BR0200504 | ۲۰۰۰ rxn of 50 µl | ۴۰ × ۱٫۲۵ ml Green Hot Start PCR Master Mix |
۱۲
UPstart™ Taq Antibody, 1 mg/ml
Features
.Inhibition of >95% Taq activity at 45°C
.۲۰۰ ng UPstart Taq Antibody are blocking 1 U Taq DNA polymerase
.Exceptionally pure made in cell culture
Applications
.Thermolabile inhibition of Taq DNA polymerases
.Convenient hot-start PCR setup at room temperature
.Fast polymerase activation with the first PCR denaturation step
| Purchase UPstart™ Taq Antibody, 1 mg/ml | ||
| Catalog # | Size | Package Content |
| BR1200101 | ۱۰۰ µg (500 U) | ۱۰۰ µl UPstart Taq Antibody |
| BR1200102 | ۲۵۰ µg (1250 U) | ۲۵۰ µl UPstart Taq Antibody |
| BR1200103 | ۱۰۰۰ µg (5000 U) | ۱ ml UPstart Taq Antibody |