Cytokeratin (HMW) Antibody (Immunohistochemistry)

🧪 Cytokeratin (HMW) Antibody (Immunohistochemistry)

The Cytokeratin (HMW) Antibody is a widely used immunohistochemical marker for identifying squamous and ductal epithelia and their related carcinomas.

This monoclonal antibody (clone 34βE12) specifically reacts with high molecular weight cytokeratins including CK1, CK5, CK10, and CK14, corresponding to molecular weights of 68, 58, 56.5, and 50 kDa. These cytokeratins are typically expressed in complex epithelia, making this antibody highly valuable for differential diagnosis of epithelial-origin carcinomas.

🔬 Features and Benefits

• Specific epithelial marker: Ideal for detecting squamous and ductal epithelial cells

• Diagnostic precision: Useful in identifying carcinomas of the prostate, breast, and other epithelial tissues

• Clear cytoplasmic staining: Brown staining observed in the cytoplasm of positive cells

• Compatible with standard IHC protocols: Suitable for both clinical and research laboratories

• Stable performance: Long shelf life (18 months) at 2–8 °C

⚙️ Principle of the Assay

During the IHC process, the Cytokeratin (HMW) antibody binds to its target antigen within the tissue. A secondary antibody conjugated with HRP attaches to the primary antibody, forming the antigen–antibody–HRP complex. When DAB chromogen is added, a brown insoluble staining reaction occurs in the cytoplasm of positive cells, confirming cytokeratin expression.

💡 Diagnostic Applications

• Detection of squamous and ductal carcinomas

• Identification of complex epithelial neoplasms

• Differential diagnosis of prostate carcinoma and other tissue tumors

• Research on HMW cytokeratin expression in pathology and molecular biology

🌐 Fardad Azma Rad Co.

Fardad Azma Rad Co., the exclusive representative of Talent Biomedical in Iran, supplies high-quality immunohistochemistry antibodies, including Cytokeratin (HMW).
With a strong commitment to accuracy, quality, and international diagnostic standards, Fardad Azma Rad delivers advanced molecular and histopathological solutions for laboratories and research centers across Iran.

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