Ligases & NA Modifying Enzymes
T4 DNA Ligase Rapid, 600 U/µl
Features
.Exceptionally pure high-concentration T4 DNA Ligase for demanding applications
.Rapid ligation
.Supplied with two buffers for fast and usual ligation protocols
Applications
.Blunt and sticky end DNA ligation for cloning
.Joining of linkers or adaptors to double-stranded DNA
.Self-circularization of linear DNA
| Purchase T4 DNA Ligase Rapid, 600 U/µl | ||
| Catalog # | Size | Package Content |
| BR1100301 | 60000 U | 100 rxn | 100 µl T4 DNA Ligase Rapid 500 µl 2× Rapid Ligation Buffer 500 µl 10× Ligation Buffer |
| BR1100302 | 180000 U | 300 rxn | 3 × 100 µl T4 DNA Ligase Rapid 3 × 500 µl 2× Rapid Ligation Buffer 3 × 500 µl 10× Ligation Buffer |
Uracil-DNA Glycosylase, 2 U/µl
Features
.Exceptionally pure Uracil–DNA Glycosylase for demanding applications
.High value for a fair price
Applications
.PCR carry-over contamination control
.Site-directed mutagenesis
.Glycosylase-mediated single-nucleotide polymorphism detection
| Purchase Uracil-DNA Glycosylase, 2 U/µl | ||
| Catalog # | Size | Package Content |
| BR1100401 | 200 U | 200 rxn | 100 µl Uracil-DNA Glycosylase 300 µl 10× UDG Reaction Buffer |
| BR1100402 | 1000 U | 1000 rxn | 5 × 100 µl Uracil-DNA Glycosylase 5 × 300 µl 10× UDG Reaction Buffer |
Shrimp Alkaline Phosphatase (rSAP), 1 U/µl
Features
.Quickly and easily removes 5′ phosphates from DNA, RNA, dNTPs and proteins
.Completely inactivated after 5 min at 65°C
.Active in common restriction enzyme and PCR buffers
.Exhibits excellent stability at 4°C and room temperature
Applications
.Removal of nucleotides from PCR prior to sequencing
.Dephosphorylation of restriction-digested vectors to prevent religation prior to cloning
.Rapid dephosphorylation of RNA, proteins or other biomolecules
| Purchase Shrimp Alkaline Phosphatase (rSAP), 1 U/µl | ||
| Catalog # | Size | Package Content |
| BR1100601 | 500 U | 500 µl Shrimp Alkaline Phosphatase |
| BR1100602 | 2500 U | 5 × 500 µl Shrimp Alkaline Phosphatase |
Heat Labile Uracil-DNA Glycosylase, 1 U/µl
Features
.The only Uracil–DNA glycosylase that is completely and irreversibly heat inactivated
.Heat-labile without any addition of agents or inhibitors
.Active in common PCR and RT-PCR buffers
Applications
.Eliminates carry-over contamination in PCR, RT-PCR, qPCR and RT-qPCR
.Enables downstream post-PCR analysis such as cloning and sequencing
| Purchase Heat Labile Uracil-DNA Glycosylase, 1 U/µl | ||
| Catalog # | Size | Package Content |
| BR1100701 | 100 U | 400 rxn | 100 µl Heat Labile Uracil-DNA Glycosylase |
| BR1100702 | 500 U |2000 rxn | 5 × 100 µl Heat Labile Uracil-DNA Glycosylase |
Heat Labile dsDNase, 2 U/µl
Features
.Provides endonuclease activity specific for double-stranded DNA
.Is easily heat-inactivated at low temperatures
.Degrades genomic DNA in RNA preps
.Does not affect RNA quality or quantity
Applications
.Easy integration into cDNA synthesis protocols
.Removal of genomic DNA from RNA preparations
.Removal of contaminating DNA from PCR, qPCR and RT-qPCR
.Purification of DNA polymerases
| Purchase Heat Labile dsDNase, 2 U/µl | ||
| Catalog # | Size | Package Content |
| BR1100801 | 100 U | 50 rxn | 50 µl Heat Labile dsDNase |
| BR1100802 | 500 U |250 rxn | 5 × 50 µl Heat Labile dsDNase |