Reverse Transcription & RT-PCR
RevertUP™ II Reverse Transcriptase, 200 U/µl
Features
.Improved performance for synthesis of long cDNAs (≥ 19 kb)
.Excellent stability at high temperatures up to 60°C
.High sensitivity for cDNA synthesis from few copies of template
Applications
.First-strand cDNA synthesis
.Generation of labeled cDNA
.RNA analysis by primer extension
.cDNA library construction
.RT-PCR
| Purchase RevertUP™ II Reverse Transcriptase, 200 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400501 | 10000 U | 50 rxn | 50 µl RevertUP II Reverse Transcriptase 1 ml 5× RevertUP Buffer |
| BR0400502 | 50000 U | 250 rxn | 2 × 125 µl RevertUP II Reverse Transcriptase 2 × 1 ml 5× RevertUP Buffer |
MMuLV Reverse Transcriptase, 200 U/µl
Features
.Pure reverse transcriptase for cDNA synthesis
.High yields of first-strand cDNA
.High value for a fair price
Applications
.First-strand cDNA synthesis
.Generation of labeled cDNA
.RNA analysis by primer extension
| Purchase MMuLV Reverse Transcriptase, 200 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400201 | 10000 U | 50 rxn | 50 µl MMuLV Reverse Transcriptase 100 µl 10× MMuLV RT Buffer |
| BR0400202 | 50000 U | 250 rxn | 5 × 50 µl MMuLV Reverse Transcriptase 5 × 100 µl 10× MMuLV RT Buffer |
One Step RT PCR Kit
Features
.Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
.Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
.PCR enhancers allowing sensitive low background amplification
Applications
.One-step RT-PCR
.Virus detection
.Amplification of GC-rich and complex templates
| Purchase One Step RT PCR Kit | ||
| Catalog # | Size | Package Content |
| BR0400101 | 50 rxn of 50 µl | 1.25 ml One Step Mix 125 µl RT-RI Blend |
| BR0400102 | 100 rxn of 50 µl | 2 × 1.25 ml One Step Mix 2 × 125 µl RT-RI Blend |
| BR0400103 | 500 rxn of 50 µl | 10 × 1.25 ml One Step Mix 10 × 125 µl RT-RI Blend |
cDNA Synthesis Kit
Features
.Highly efficient synthesis of long cDNAs (≥ 19 kb)
.Excellent yields at temperatures up to 55°C
.High sensitivity reverse transcription from low abundance template
.Superior performance in demanding applications, including templates with a high degree of secondary structure
Applications
.First-strand cDNA synthesis for RT-PCR and qPCR
.Gene expression profiling
.RNA labeling and primer extension
.cDNA library construction
| Purchase cDNA Synthesis Kit | ||
| Catalog # | Size | Package Content |
| BR0400401 | 10 rxn of 20 µl | 10 µl Reverse Transcriptase 100 µl 5× cDNA Synthesis Buffer 20 µl dNTP Mix, 5 µl RNase Inhibitor 10 µl Hexamer and 5 µl Oligo (dT) Primer 1.5 ml PCR Grade Water |
| BR0400403 | 125 rxn of 20 µl | 125 µl Reverse Transcriptase 1 ml 5× cDNA Synthesis Buffer 250 µl dNTP Mix, 62.5 µl RNase Inhibitor 125 µl Hexamer and 62.5 µl Oligo (dT) Primer |
RNase Inhibitor, 40 U/µl
Features
.Exceptionally pure proprietary Ribonuclease Inhibitor for demanding RNA applications
.Active under variety of reaction conditions used for work with RNA
.Prevention of RNA from degradation by a wide range of RNases
Applications
.In vitro transcription/translation
.cDNA synthesis
.RNA purification and storage
| Purchase RNase Inhibitor, 40 U/µl | ||
| Catalog # | Size | Package Content |
| BR0400901 | 2500 U | 125 rxn | 62.5 µl RNase Inhibitor |
| BR0400902 | 10000 U | 500 rxn | 4 × 62.5 µl RNase Inhibitor |