E-Cadherin Antibody (Immunohistochemistry)

🧪 E-Cadherin Antibody (Immunohistochemistry)

The E-Cadherin Antibody is an essential immunohistochemical marker used for evaluating cell adhesion and tumor invasiveness.
E-Cadherin is a 120 kDa transmembrane glycoprotein expressed in epithelial cells and is responsible for cell-to-cell adhesion through its interaction with catenins.

Loss or reduction of E-Cadherin expression correlates with increased tumor invasion, metastasis, and malignancy progression, especially in gastric, breast, prostate, and ovarian carcinomas. Therefore, E-Cadherin IHC staining is a key diagnostic tool for distinguishing well-differentiated epithelial carcinomas from metastatic tumors.

🔬 Features and Benefits

• Specific marker for epithelial cell adhesion: Detects alterations in intercellular junctions

• Clear membrane staining: Brown staining visible along the cell membrane

• Broad diagnostic applications: Particularly useful in breast and gastric carcinoma diagnosis

• Compatible with standard IHC protocols

• Excellent stability: Reliable up to 18 months at 2–8 °C

⚙️ Principle of the Assay

During the IHC process, E-Cadherin antibody binds to its antigen located on epithelial cell membranes.
The HRP-conjugated secondary antibody forms the antigen–antibody–HRP complex, which reacts with DAB chromogen, producing a brown insoluble stain on the membrane of positive cells.

💡 Diagnostic Applications

• Evaluation of cell adhesion loss in epithelial tumors

• Differentiation between ductal and lobular breast carcinoma

• Identification of gastric and prostatic carcinoma

• Research studies on metastasis and epithelial cell biology

🌐 Fardad Azma Rad Co.

Fardad Azma Rad Co., the exclusive representative of Talent Biomedical in Iran, distributes high-quality immunohistochemistry antibodies, including E-Cadherin.
With a focus on accuracy, innovation, and international diagnostic standards, the company supports pathology laboratories and research centers across Iran.

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