{"id":1408,"date":"2025-05-19T10:18:16","date_gmt":"2025-05-19T06:48:16","guid":{"rendered":"https:\/\/fardadazma.com\/en\/?post_type=product&#038;p=1408"},"modified":"2025-12-01T14:59:42","modified_gmt":"2025-12-01T11:29:42","slug":"one-step-rt-pcr-master-mix","status":"publish","type":"product","link":"https:\/\/fardadazma.com\/en\/product\/one-step-rt-pcr-master-mix\/","title":{"rendered":"One-Step RT-PCR Master Mix"},"content":{"rendered":"<h2 data-start=\"196\" data-end=\"297\"><strong>One-Step RT-PCR Kit from Biotech Rabbit Germany | Ideal for Viral Detection and Complex Templates<\/strong><\/h2>\n<p data-start=\"299\" data-end=\"543\">The <strong data-start=\"303\" data-end=\"346\">One-Step RT-PCR Kit from <a href=\"https:\/\/www.biotechrabbit.com\/\">Biotech Rabbit<\/a><\/strong> is a high-quality product from the well-known German brand <strong data-start=\"406\" data-end=\"424\">Biotech Rabbit<\/strong>, designed for simultaneous reverse transcription and PCR in a single tube \u2013 ideal for rapid and reliable RNA analysis.<\/p>\n<h2 data-start=\"545\" data-end=\"562\"><strong data-start=\"545\" data-end=\"562\">Key Features One-Step RT-PCR Master Mix:<\/strong><\/h2>\n<ul data-start=\"564\" data-end=\"976\">\n<li data-start=\"564\" data-end=\"695\">\n<p data-start=\"566\" data-end=\"695\">Contains <strong data-start=\"575\" data-end=\"612\">heat-stable reverse transcriptase<\/strong> and <strong data-start=\"617\" data-end=\"636\">RNase inhibitor<\/strong> for efficient and consistent high-yield <strong data-start=\"677\" data-end=\"695\">cDNA synthesis<\/strong><\/p>\n<\/li>\n<li data-start=\"696\" data-end=\"819\">\n<p data-start=\"698\" data-end=\"819\">Includes <strong data-start=\"707\" data-end=\"739\">Hot-start Taq DNA Polymerase<\/strong> combined with <strong data-start=\"754\" data-end=\"775\">high-purity dNTPs<\/strong> to ensure high fidelity and reproducibility<\/p>\n<\/li>\n<li data-start=\"820\" data-end=\"976\">\n<p data-start=\"822\" data-end=\"976\">Enhanced with <strong data-start=\"836\" data-end=\"861\">special PCR additives<\/strong> for <strong data-start=\"866\" data-end=\"899\">ultra-sensitive amplification<\/strong> with minimal background noise, even for GC-rich or complex genetic templates<\/p>\n<\/li>\n<\/ul>\n<h3 data-start=\"978\" data-end=\"995\"><strong data-start=\"978\" data-end=\"995\">Applications One-Step RT-PCR Master Mix:<\/strong><\/h3>\n<ul data-start=\"997\" data-end=\"1166\">\n<li data-start=\"997\" data-end=\"1030\">\n<p data-start=\"999\" data-end=\"1030\"><a href=\"https:\/\/www.biotechrabbit.com\/catalogsearch\/result\/?q=One-step+RT-PCR\"><strong data-start=\"999\" data-end=\"1018\">One-step RT-PCR<\/strong><\/a> reactions<\/p>\n<\/li>\n<li data-start=\"1031\" data-end=\"1102\">\n<p data-start=\"1033\" data-end=\"1102\"><strong data-start=\"1033\" data-end=\"1080\">Rapid and accurate detection of RNA viruses<\/strong> in clinical samples<\/p>\n<\/li>\n<li data-start=\"1103\" data-end=\"1166\">\n<p data-start=\"1105\" data-end=\"1166\"><strong data-start=\"1105\" data-end=\"1166\">Robust amplification of GC-rich and complex RNA templates<\/strong><\/p>\n<\/li>\n<\/ul>\n<p data-start=\"1168\" data-end=\"1300\">\ud83e\uddea This kit is <strong data-start=\"1183\" data-end=\"1233\">certified with international quality standards<\/strong> and is widely used in advanced molecular diagnostics laboratories.<\/p>\n<p data-start=\"1302\" data-end=\"1481\">\ud83d\udccc <strong data-start=\"1305\" data-end=\"1328\"><a href=\"https:\/\/fardadazma.com\/about\/\">Fardad Azma Rad<\/a> Co.<\/strong> is the <strong data-start=\"1336\" data-end=\"1379\">exclusive distributor of Biotech Rabbit<\/strong> in Iran, providing official access to this kit and other molecular diagnostic products in the region.<\/p>\n<h3><a style=\"font-size: 16px\" href=\"\/wp-content\/uploads\/2025\/02\/PIN-04001-003_1-Step_RT_PCR_Master_Mix.pdf\"><strong>Download Catalog<\/strong><\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"<h3><strong>Overview:<\/strong><\/h3>\n<p><strong>Biotechrabbit\u2122 1-Step RT-PCR Mix<\/strong>\u00a0offers an easy and efficient solution for simultaneous reverse transcription (RT) of RNA and PCR amplification of cDNA in a single step. Simply add RNA samples, primers, and PCR water to proceed.<\/p>\n<h3><strong>Key Components:<\/strong><\/h3>\n<ul>\n<li><strong>RT-RI 20\u00d7 Mix:<\/strong>\u00a0Contains stable reverse transcriptase able to operate at different temperatures, along with a specific RNase inhibitor, ensuring high-performance cDNA synthesis.<\/li>\n<li><strong>2X 1-Step Master Mix:<\/strong>\u00a0Combines hot-start Taq DNA Polymerase, high-quality dNTPs, MgCl\u2082, and stabilizing agents in an optimized buffer. It produces high yields of PCR products with minimal background, even from low-abundance or challenging templates.<\/li>\n<\/ul>\n<h3><strong>Benefits:<\/strong><\/h3>\n<ul>\n<li>Sensitive detection of difficult or GC-rich targets<\/li>\n<li>Minimal background noise for high-specificity results<\/li>\n<li>Rapid, one-tube workflow suitable for diagnostic labs, research, and pathogen detection<\/li>\n<\/ul>\n","protected":false},"featured_media":1406,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[28],"product_tag":[210,246,243,248,250,253,251,249,245,252,244,236,172,247],"class_list":{"0":"post-1408","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-genetics","7":"product_tag-cdna-synthesis","8":"product_tag-diagnostic-pcr","9":"product_tag-easy-pcr-setup","10":"product_tag-gc-rich-template-amplification","11":"product_tag-high-temperature-reverse-transcriptase","12":"product_tag-one-step-rt-pcr","13":"product_tag-pcr-amplification","14":"product_tag-pcr-enhancers","15":"product_tag-real-time-pcr","16":"product_tag-reverse-transcription","17":"product_tag-rna-to-dna-conversion","18":"product_tag-rnase-inhibitor","19":"product_tag-taq-dna-polymerase","20":"product_tag-virus-detection","22":"first","23":"instock","24":"shipping-taxable","25":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - 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