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Lactose Broth

Blood Agar Base

Lactose Broth

Attributes

  • product-brand: Biolab

Lactose Broth

Lactose Broth is a liquid biochemical culture medium designed to detect the ability of bacteria to ferment lactose and produce gas.
This product is manufactured by BioLab and is exclusively imported and distributed in Iran by Fardad Azma Rad Co.

It is a standard medium used in microbiology to study the fermentation characteristics of various bacteria.

Key Features and Composition:

  • Lactose:
    The primary carbohydrate in the medium; its fermentation leads to acid and sometimes gas production, indicating metabolic activity.

  • Peptones:
    Serve as a source of amino acids and nitrogen, supporting bacterial growth and metabolic functions.

  • Salts:
    Help maintain osmotic balance and stabilize the pH of the medium, which is essential for accurate biochemical reactions.

  • Phenol Red Indicator:
    A pH indicator that turns yellow in the presence of acid, signaling lactose fermentation by the organism.

  • Gas Detection Capability:
    When used with a Durham tube, gas production during fermentation is visible as trapped gas bubbles.

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Main Applications of Lactose Broth

  1. Identification and Differentiation of Bacteria Based on Lactose Fermentation: Lactose Broth is primarily used to identify lactose-fermenting bacteria. Many enteric bacteria, such as Escherichia coli, can ferment lactose, and this medium is highly effective for identifying them.

  2. Enteric Bacteria Detection: This medium is used to identify enteric bacteria, particularly for diagnosing gastrointestinal infections. Bacteria such as E. coli, Enterobacter, and Klebsiella, which ferment lactose, can be easily identified in this medium.

  3. Gas Production Test: Many bacteria produce gas when fermenting lactose. This gas is typically seen as small bubbles in the medium, and this feature is particularly useful for identifying lactose-fermenting bacteria.

  4. Urinary and Gastrointestinal Infections: Lactose Broth is used in clinical microbiology laboratories for identifying bacteria associated with urinary and gastrointestinal infections. It is particularly useful for diagnosing Escherichia coli infections, which are common in urinary tract infections.

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